Solution Information | help | |
Enzyme: | G-protein coupled receptor 55 | |
inhibitor: | BDBM33070 | |
substrate: | n/a | |
Solution Type: | Aqueous | |
pH at Preparation: | n/a | |
Temp. Prep.: | n/a | |
Comments: | Assay Materials: 1) 384-well plates, black with clear bottom (Greiner# 781091) 2) U2OS (Human Osteosarcoma) cell line stably expressing the Beta-arrestin GFP and GPR55 receptor 3) Culture Media: MEM with L-glutamine, Pen-strep, 10% Fetal Bovine Serum and selection antibiotics - 100ug/ml G418 and 50ug/ml Zeocin 4) Positive Control Working Solution: Lysophosphatidylinositol (Sigma L7635, 5mM stock in Methanol water) diluted in water to 250uM. Additional DMSO is added to achieve a DMSO concentration of 2.5%. 5) Negative Control Working Solution: 2.5% DMSO diluted in water. 6) Fixative Working Solution: 6% Paraformaldehyde (PFA) diluted in PBS. 7) Nuclear Stain Working Solution: DAPI (Invitrogen, D1306) diluted to 150ng/ml in DAPI buffer (10mM TRIS, 10mM EDTA, 100mM NaCl, pH 7.4). Primary Screen Procedure: 1) 45ul of cell suspension (200,000 cells/ml in culture medium) was dispensed in each well of the assay plates using a Wellmate bulk dispenser. 2) Plates are incubated overnight or | |
If you find an error in this entry please send us an E-mail |
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |